Target：sMtCK

Fields：Arginine and proline metabolism;Metabolic pathways

Gene Name：CKMT2

Protein Name：Creatine kinase S-type mitochondrial

Human Gene Id：1160

Human Swiss Prot No：P17540

Mouse Gene Id：76722

Mouse Swiss Prot No：Q6P8J7

Rat Swiss Prot No：P09605

Immunogen：The antiserum was produced against synthesized peptide derived from human CKMT2. AA range:231-280

Specificity：sMtCK Polyclonal Antibody detects endogenous levels of sMtCK protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CKMT2;Creatine kinase S-type; mitochondrial;Basic-type mitochondrial creatine kinase;Mib-CK;Sarcomeric mitochondrial creatine kinase;S-MtCK

Observed Band(KD)：48kD

Background：creatine kinase, mitochondrial 2(CKMT2) Homo sapiens Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gen

Function：catalytic activity:ATP + creatine = ADP + phosphocreatine.,function:Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.,miscellaneous:Mitochondrial creatine kinase binds cardiolipin.,similarity:Belongs to the ATP:guanido phosphotransferase family.,subunit:Exists as an octamer composed of four CKMT2 homodimers.,tissue specificity:Sarcomere-specific. Found only in heart and skeletal muscles.,

Subcellular Location：Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Expression：Sarcomere-specific. Found only in heart and skeletal muscles.