Target：SMIF

Fields：RNA degradation

Gene Name：DCP1A

Protein Name：mRNA-decapping enzyme 1A

Human Gene Id：55802

Human Swiss Prot No：Q9NPI6

Mouse Gene Id：75901

Mouse Swiss Prot No：Q91YD3

Immunogen：The antiserum was produced against synthesized peptide derived from human DCP1A. AA range:111-160

Specificity：SMIF Polyclonal Antibody detects endogenous levels of SMIF protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：DCP1A;SMIF;mRNA-decapping enzyme 1A;Smad4-interacting transcriptional co-activator;Transcription factor SMIF

Observed Band(KD)：75kD

Background： Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014],

Function：function:Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.,similarity:Belongs to the DCP1 family.,subcellular location:Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.,subunit:Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Binds DCP1B, UPF1 and SMAD4. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with PNRC2.,tissue specificity:Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.,

Subcellular Location：Cytoplasm, P-body . Nucleus . Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524). .

Expression：Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.