Target：HisRS
Fields：Aminoacyl-tRNA biosynthesis
Gene Name：HARS
Protein Name：Histidine--tRNA ligase cytoplasmic
Human Gene Id：3035
Human Swiss Prot No：P12081
Mouse Gene Id：15115
Mouse Swiss Prot No：Q61035
Immunogen：The antiserum was produced against synthesized peptide derived from human HARS. AA range:460-509
Specificity：HisRS Polyclonal Antibody detects endogenous levels of HisRS protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：HARS;HRS;Histidine--tRNA ligase; cytoplasmic;Histidyl-tRNA synthetase;HisRS
Observed Band(KD)：60kD
Background： Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012],
Function：catalytic activity:ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,similarity:Contains 1 WHEP-TRS domain.,tissue specificity:Brain, heart, liver and kidney.,
Subcellular Location：Cytoplasm .
Expression：Brain, heart, liver and kidney.
