Target：GSTT1/4

Fields：Glutathione metabolism;Metabolism of xenobiotics by cytochrome P450;Drug metabolism - cytochrome P450;Drug metabolism - other enzymes;Metabolic pathways;Platinum drug resistance;Pathways in cancer;Chemical carcinogenesis - DNA adducts;Chemical carcinogenesis - receptor activation;Chemical carcinogenesis - reactive oxygen species;Hepatocellular carcinoma;Fluid shear stress and atherosclerosis

Gene Name：GSTT1/GSTT4

Protein Name：Glutathione S-transferase theta-1/Glutathione S-transferase theta-4

Human Gene Id：2952

Human Swiss Prot No：P30711/A8MPT4

Immunogen：The antiserum was produced against synthesized peptide derived from human GSTT1/4. AA range:10-59

Specificity：GSTT1/4 Polyclonal Antibody detects endogenous levels of GSTT1/4 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：GSTT1;Glutathione S-transferase theta-1;GST class-theta-1;Glutathione transferase T1-1;GSTT4;Glutathione S-transferase theta-4;GST class-theta-4

Observed Band(KD)：30kD

Background： The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015],

Function：catalytic activity:RX + glutathione = HX + R-S-glutathione.,function:Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.,online information:The Singapore human mutation and polymorphism database,polymorphism:The GSTT1 gene is absent from 38% of the population. The presence or absence of the GSTT1 gene is coincident with the conjugator (GSST1+) and non-conjugator (GSTT1-) phenotypes respectively. The GSTT1+ phenotype can catalyze the glutathione conjugation of dichloromethane.,similarity:Belongs to the GST superfamily. Theta family.,similarity:Contains 1 GST C-terminal domain.,similarity:Contains 1 GST N-terminal domain.,subunit:Homodimer.,tissue s

Subcellular Location：Cytoplasm.

Expression：Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.