Catalog No.
QYA05769A
Size.
100ug/100ul
Source.
Mouse
Immunogen.
Recombinant Protein of mCherry-Tag
Purification.
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using specific immunogen.
Specificity.
The antibody detects mCherry and mCherry tag fusion proteins.
Formulation.
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Concentration.
1mg/ml
Storage / Stability.
-20°C/1 year
Reactivity.
Species independent
Applications.
WB
Dilution.
WB: 1:5000 -10000
Background .
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Western blot analysis of mCherry Recombinant protein, diluted at 1) 1:5000 2) 1:20000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000