Target：Clusterin

Fields：Complement and coagulation cascades

Gene Name：CLU

Protein Name：Clusterin

Human Gene Id：1191

Human Swiss Prot No：P10909

Mouse Gene Id：12759

Mouse Swiss Prot No：Q06890

Rat Gene Id：24854

Rat Swiss Prot No：P05371

Immunogen：The antiserum was produced against synthesized peptide derived from human CLU. AA range:400-449

Specificity：Clusterin Polyclonal Antibody detects endogenous levels of Clusterin protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CLU;APOJ;CLI;KUB1;AAG4;Clusterin;Aging-associated gene 4 protein;Apolipoprotein J;Apo-J;Complement cytolysis inhibitor;CLI;Complement-associated protein SP-40;40;Ku70-binding protein 1;NA1/NA2;Testosterone-repressed prostate m

Observed Band(KD)：52kD

Background： The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011],

Function：function:Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death (apoptosis).,similarity:Belongs to the clusterin family.,subunit:Antiparallel disulfide-linked heterodimer. Interacts with APOA1, CLUAP1 AND PON1.,

Subcellular Location：[Isoform 1]: Secreted . Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. .; [Isoform 4]: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; [Isoform 6]: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; Nucleus . Cytoplasm . Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm, cytosol . Microsome . Endoplasmic reticulum . Mitochondrion . Mitochondrion membrane . Cytoplasm, perinuclear region . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress (PubMed:17451556). Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated

Expression：Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.