Target：CLASP1

Gene Name：CLASP1

Protein Name：CLIP-associating protein 1

Human Gene Id：23332

Human Swiss Prot No：Q7Z460

Mouse Gene Id：76707

Mouse Swiss Prot No：Q80TV8

Immunogen：The antiserum was produced against synthesized peptide derived from human CLASP1. AA range:1171-1220

Specificity：CLASP1 Polyclonal Antibody detects endogenous levels of CLASP1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CLASP1;KIAA0622;MAST1;CLIP-associating protein 1;Cytoplasmic linker-associated protein 1;Multiple asters homolog 1;Protein Orbit homolog 1;hOrbit1

Observed Band(KD)：169kD

Background：cytoplasmic linker associated protein 1(CLASP1) Homo sapiens CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008],

Function：function:Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the CLASP family.,similarity:Contains 7 HEAT repeats.,subcellular location:Localizes to microtubule plus ends. Localizes to centrosomes, kinetochor

Subcellular Location：Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golgi network . Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.

Expression： Brain,Endometrium carcinoma cell line,Epithelium,Eye,Platelet,Poole