Target：c-FLIP

Fields：NF-kappa B signaling pathway;Autophagy - animal;Apoptosis;Necroptosis;TNF signaling pathway;Chagas disease;Hepatitis C

Gene Name：CFLAR

Protein Name：CASP8 and FADD-like apoptosis regulator

Human Gene Id：8837

Human Swiss Prot No：O15519

Mouse Swiss Prot No：O35732

Immunogen：The antiserum was produced against synthesized peptide derived from the N-terminal region of human CFLAR. AA range:1-50

Specificity：c-FLIP Polyclonal Antibody detects endogenous levels of c-FLIP protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CFLAR;CASH;CASP8AP1;CLARP;MRIT;CASP8 and FADD-like apoptosis regulator;Caspase homolog;CASH;Caspase-eight-related protein;Casper;Caspase-like apoptosis regulatory protein;CLARP;Cellular FLICE-like inhibitory protein;c-FLIP;FADD-like antiapoptotic molecule 1;FLAME-1;Inhibitor of FLICE;I-FLICE;MACH-related inducer of toxicity;MRIT;Usurpin

Observed Band(KD)：55kD

Background： The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011],

Function：domain:The caspase domain lacks the active sites residues involved in catalysis.,function:Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.,induction:Repressed by IL-2 after TCR stimulation, during progression to the S-phase of the cell cycle.,PTM:Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.,similarity:Belongs to the peptidase C14A family.,similarity:Contains

Subcellular Location：cytoplasm,cytosol,death-inducing signaling complex,CD95 death-inducing signaling complex,membrane raft,ripoptosome,

Expression：Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.