Target：Cdk4

Fields：Endocrine resistance;Cell cycle;p53 signaling pathway;PI3K-Akt signaling pathway;Cellular senescence;Tight junction;T cell receptor signaling pathway;AGE-RAGE signaling pathway in diabetic complications;Cushing syndrome;Hepatitis C;Measles;Human cytomegalovirus infection;Influenza A;Human papillomavirus infection;Human T-cell leukemia virus 1 infection;Kaposi sarcoma-associated herpesvirus infection;Epstein-Barr virus infection;Pathways in cancer;Viral carcinogenesis;Pancreatic cancer;Glioma;Melanoma;Bladder cancer;Chronic myeloid leukemia;Small cell lung cancer;Non-small cell lung cancer;Breast cancer;Hepatocellular carcinoma

Gene Name：CDK4

Protein Name：Cyclin-dependent kinase 4

Human Gene Id：1019

Human Swiss Prot No：P11802

Mouse Gene Id：12567

Mouse Swiss Prot No：P30285

Rat Gene Id：94201

Rat Swiss Prot No：P35426

Immunogen：The antiserum was produced against synthesized peptide derived from the N-terminal region of human CDK4. AA range:1-50

Specificity：Cdk4 Polyclonal Antibody detects endogenous levels of Cdk4 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IF 1:50-200 WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CDK4;Cyclin-dependent kinase 4;Cell division protein kinase 4;PSK-J3

Observed Band(KD)：35kD

Background：cyclin dependent kinase 4(CDK4) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:CDK4 mutations are involved in tumor formation.,disease:Defects in CDK4 are the cause of cutaneous malignant melanoma 3 (CMM3) [MIM:609048, 155600]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a preexisting benign nevus, which occurs most often in the skin but also may involve other sites.,enzyme regulation:Phosphorylation at Thr-172 is necessary for enzymatic activity.,function:Probably involved in the control of the cell cycle.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable complex with D-type G1 cyclins. Interacts with SEI1 and ZNF655/VIK.,

Subcellular Location：Cytoplasm . Nucleus . Nucleus membrane . Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. .

Expression： Brain,Muscle,