Target：Rock-2

Fields：cGMP-PKG signaling pathway;cAMP signaling pathway;Chemokine signaling pathway;Sphingolipid signaling pathway;Vascular smooth muscle contraction;Wnt signaling pathway;Axon guidance;Focal adhesion;Tight junction;Platelet activation;Leukocyte transendothelial migration;Regulation of actin cytoskeleton;Oxytocin signaling pathway;Pathogenic Escherichia coli infection;Shigellosis;Salmonella infection;Yersinia infection;Human cytomegalovirus infection;Pathways in cancer;Proteoglycans in cancer;Lipid and atherosclerosis

Gene Name：ROCK2

Protein Name：Rho-associated protein kinase 2

Human Gene Id：9475

Human Swiss Prot No：O75116

Mouse Gene Id：19878

Mouse Swiss Prot No：P70336

Rat Gene Id：25537

Rat Swiss Prot No：Q62868

Immunogen：The antiserum was produced against synthesized peptide derived from human ROCK2. AA range:691-740

Specificity：Rock-2 Polyclonal Antibody detects endogenous levels of Rock-2 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunocytochemistry: 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ROCK2;KIAA0619;Rho-associated protein kinase 2;Rho kinase 2;Rho-associated; coiled-coil-containing protein kinase 2;Rho-associated, coiled-coil-containing protein kinase II;ROCK-II;p164 ROCK-2

Observed Band(KD)：165kD

Background： The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by RHOA binding.,function:Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.,miscellaneous:Inhibited by Y-27632.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 REM (Hr1) repeat.,subcellular location:Cytoplasmic, and associated with actin microfilaments and the plasma membrane.,subunit:Homodim

Subcellular Location：Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. .

Expression：Expressed in the brain (at protein level).