Target：CD148

Fields：Adherens junction;Chemical carcinogenesis - reactive oxygen species

Gene Name：PTPRJ

Protein Name：Receptor-type tyrosine-protein phosphatase eta

Human Gene Id：5795

Human Swiss Prot No：Q12913

Mouse Gene Id：19271

Mouse Swiss Prot No：Q64455

Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human PTPRJ. AA range:861-910

Specificity：CD148 Polyclonal Antibody detects endogenous levels of CD148 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PTPRJ;DEP1;Receptor-type tyrosine-protein phosphatase eta;Protein-tyrosine phosphatase eta;R-PTP-eta;Density-enhanced phosphatase 1;DEP-1;HPTP eta;Protein-tyrosine phosphatase receptor type J;R-PTP-J;CD148

Observed Band(KD)：150kD

Background： The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms

Function：catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,disease:Defects in PTPRJ are found in cancers of colon, lung, and breast.,function:May contribute to the mechanism of contact inhibition of cell growth.,PTM:N- and O-glycosylated.,similarity:Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,similarity:Contains 9 fibronectin type-III domains.,

Subcellular Location：Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane . Cell junction. After T-cell stimulation, it is temporarily excluded from immunological synapses.

Expression：Expressed in the promyelocytic cell line HL-60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the IL3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.