Target：MAN1B1

Fields：N-Glycan biosynthesis;Various types of N-glycan biosynthesis;Metabolic pathways;Protein processing in endoplasmic reticulum

Gene Name：MAN1B1

Protein Name：Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Human Gene Id：11253

Human Swiss Prot No：Q9UKM7

Mouse Swiss Prot No：A2AJ15

Immunogen：Synthesized peptide derived from MAN1B1 . at AA range: 100-180

Specificity：MAN1B1 Polyclonal Antibody detects endogenous levels of MAN1B1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MAN1B1;Endoplasmic reticulum mannosyl-oligosaccharide 1;2-alpha-mannosidase;ER alpha-1,2-mannosidase;ER mannosidase 1;ERMan1;Man9GlcNAc2-specific-processing alpha-mannosidase;Mannosidase alpha class 1B member 1

Observed Band(KD)：80kD

Background： This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011],

Function：catalytic activity:Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).,cofactor:Calcium.,enzyme regulation:Inhibited by both 1-deoxymannojirimycin and kifunensine.,function:Involved in the maturation of Asn-linked oligosaccharides. Trim a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2). The only product is the Man(8)GlcNAc(2) isomer B, the form lacking the middle-arm terminal alpha 1,2-mannose. It may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyl hydrolase 47 family.,tissue specificity:Widely expressed.,

Subcellular Location：Endoplasmic reticulum membrane ; Single-pass type II membrane protein .

Expression：Widely expressed.