Target：MAPK Organizer 1

Gene Name：WDR83

Protein Name：WD repeat domain-containing protein 83

Human Gene Id：84292

Human Swiss Prot No：Q9BRX9

Mouse Gene Id：67836

Mouse Swiss Prot No：Q9DAJ4

Rat Gene Id：288924

Rat Swiss Prot No：Q5BLX8

Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human WDR83. AA range:141-190

Specificity：MAPK Organizer 1 Polyclonal Antibody detects endogenous levels of MAPK Organizer 1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：WDR83;MORG1;WD repeat domain-containing protein 83;Mitogen-activated protein kinase organizer 1;MAPK organizer 1

Observed Band(KD)：34kD

Background： This gene encodes a member of the WD-40 protein family. The protein is proposed to function as a molecular scaffold for various multimeric protein complexes. The protein associates with several components of the extracellular signal-regulated kinase (ERK) pathway, and promotes ERK activity in response to serum or other signals. The protein also interacts with egl nine homolog 3 (EGLN3, also known as PHD3) and regulates expression of hypoxia-inducible factor 1, and has been purified as part of the spliceosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],

Function：function:Molecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. Also involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator (By similarity). May also be involved in pre-mRNA splicing.,similarity:Belongs to the WD repeat MORG1 family.,similarity:Contains 7 WD repeats.,subcellular location:Predominantly cytoplasmic (By similarity). Partially nuclear.,subunit:Interacts with EGLN3/PHD3. Interacts with ERK signaling proteins MAP2K1/MEK1, MAP2K2/MEK2, MAP2K1IP1/M

Subcellular Location：Cytoplasm . Nucleus . Predominantly cytoplasmic. Partially nuclear. .

Expression： Kidney proximal tubule,Muscle,