Target：MARCH5

Gene Name：MARCH5

Protein Name：E3 ubiquitin-protein ligase MARCH5

Human Gene Id：54708

Human Swiss Prot No：Q9NX47

Mouse Gene Id：69104

Mouse Swiss Prot No：Q3KNM2

Immunogen：The antiserum was produced against synthesized peptide derived from human MARCH5. AA range:21-70

Specificity：MARCH5 Polyclonal Antibody detects endogenous levels of 40607 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MARCH5;RNF153;E3 ubiquitin-protein ligase MARCH5;Membrane-associated RING finger protein 5;Membrane-associated RING-CH protein V;MARCH-V;Mitochondrial ubiquitin ligase;MITOL;RING finger protein 153

Observed Band(KD)：37kD

Background： MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008],

Function：domain:The RING-CH-type zinc finger domain is required for E3 ligase activity.,function:Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology. Promotes ubiquitination of DRP1. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-CH-type zinc finger.,subunit:Interacts with MTFN2 and ubiquitinated forms of DRP1.,

Subcellular Location：Mitochondrion outer membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301). .

Expression：Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.