Target：CB1

Fields：Rap1 signaling pathway;Neuroactive ligand-receptor interaction;Thermogenesis;Retrograde endocannabinoid signaling

Gene Name：CNR1

Protein Name：Cannabinoid receptor 1

Human Gene Id：1268

Human Swiss Prot No：P21554

Mouse Gene Id：12801

Mouse Swiss Prot No：P47746

Rat Gene Id：25248

Rat Swiss Prot No：P20272

Immunogen：The antiserum was produced against synthesized peptide derived from human CNR1. AA range:151-200

Specificity：CB1 Polyclonal Antibody detects endogenous levels of CB1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CNR1;CNR;Cannabinoid receptor 1;CB-R;CB1;CANN6

Observed Band(KD)：53kD

Background： This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009],

Function：function:Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Interacts (via C-terminus) with CNRIP1.,tissue specificity:Widely expressed.,

Subcellular Location：Cell membrane ; Multi-pass membrane protein . Membrane raft . Mitochondrion outer membrane . Cell projection, axon . Cell junction, synapse, presynapse . Unexpectedly, in the mitochondria, the C-terminus is located in the mitochondrial intermembrane space, a compartment topologically considered as extracellular. In canonical seven-transmembrane G-protein coupled receptors, the C-terminus is cytosolic (By similarity). Found on presynaptic axon terminals in some GABAergic neurons in the somatosensory cortex (By similarity). .

Expression：Widely expressed, with highest levels in fetal and adult brain. Expression levels of isoform 2 and isoform 3 are much lower than those of isoform 1.