Target：Casein Kinase Iδ

Fields：Hedgehog signaling pathway;Hippo signaling pathway;Gap junction;Circadian rhythm

Gene Name：CSNK1D

Protein Name：Casein kinase I isoform delta

Human Gene Id：1453

Human Swiss Prot No：P48730

Mouse Gene Id：104318

Mouse Swiss Prot No：Q9DC28

Rat Gene Id：64462

Rat Swiss Prot No：Q06486

Immunogen：The antiserum was produced against synthesized peptide derived from human CSNK1D. AA range:291-340

Specificity：Casein Kinase Iδ Polyclonal Antibody detects endogenous levels of Casein Kinase Iδ protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CSNK1D;HCKID;Casein kinase I isoform delta;CKI-delta;CKId;Tau-protein kinase CSNK1D

Observed Band(KD)：47kD

Background： This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.,enzyme regulation:Exhibits substrate-dependent heparin activation.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.,PTM:Autophosphorylated on serine and threonine residues.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr prot

Subcellular Location：Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Localized at mitotic spindle microtubules, and at the centrosomes and interphase in interphase cells. Recruited to the spindle apparatus and the centrosomes in response to DNA-damage. Correct subcellular localization requires kinase activity.

Expression：Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. However, kinase activity is not uniform, with highest kinase activity in splenocytes. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.