Target：CARD 14

Fields：NF-kappa B signaling pathway

Gene Name：CARD14

Protein Name：Caspase recruitment domain-containing protein 14

Human Gene Id：79092

Human Swiss Prot No：Q9BXL6

Mouse Gene Id：170720

Mouse Swiss Prot No：Q99KF0

Immunogen：The antiserum was produced against synthesized peptide derived from human CAR14. AA range:291-340

Specificity：CARD 14 Polyclonal Antibody detects endogenous levels of CARD 14 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CARD14;CARMA2;Caspase recruitment domain-containing protein 14;CARD-containing MAGUK protein 2;Carma 2

Observed Band(KD)：110kD

Background： This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012],

Function：caution:Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.,function:Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.,similarity:Contains 1 CARD domain.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,subunit:CARD14 and BCL10 bind to each other by CARD-CARD interaction.,tissue specificity:Expressed in placenta. Also detected in HeLa S3 cells, but not in the other cancer cell lines tested.,

Subcellular Location：[Isoform 1]: Cytoplasm .; [Isoform 2]: Cytoplasm .; [Isoform 3]: Cytoplasm .

Expression：Isoform 1 is detected in placenta and epidermal keratinocytes (PubMed:22521418). Isoform 2 is detected in leukocytes and fetal brain (PubMed:22521418).