Target：CAN1

Fields：Protein processing in endoplasmic reticulum;Apoptosis;Necroptosis;Cellular senescence;Alzheimer disease;Pathways of neurodegeneration - multiple diseases;Shigellosis

Gene Name：CAPN1 CANPL1 PIG30

Protein Name：Calpain-1 catalytic subunit (EC 3.4.22.52) (Calcium-activated neutral proteinase 1) (CANP 1) (Calpain mu-type) (Calpain-1 large subunit) (Cell proliferation-inducing gene 30 protein) (Micromolar-calpa

Human Gene Id：823

Human Swiss Prot No：P07384

Mouse Swiss Prot No：O35350

Rat Swiss Prot No：P97571

Immunogen：Synthesized peptide derived from part region of human protein

Specificity：CAN1 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：78kD

Background： The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010],

Function：catalytic activity:Broad endopeptidase specificity.,cofactor:Binds 3 calcium ions.,enzyme regulation:Activated by micromolar concentrations of calcium and inhibited by calpastatin.,function:Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.,online information:Calpain,similarity:Belongs to the peptidase C2 family.,similarity:Contains 1 calpain catalytic domain.,similarity:Contains 4 EF-hand domains.,subcellular location:Translocates to the plasma membrane upon Ca(2+) binding.,subunit:Forms a heterodimer with a small (regulatory) subunit (CAPNS1).,tissue specificity:Ubiquitous.,

Subcellular Location：Cytoplasm . Cell membrane . Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719). .

Expression：Ubiquitous.