Target：RNase III Drosha

Fields：Ribosome biogenesis in eukaryotes;Proteoglycans in cancer

Gene Name：DROSHA

Protein Name：Ribonuclease 3

Human Gene Id：29102

Human Swiss Prot No：Q9NRR4

Mouse Gene Id：14000

Mouse Swiss Prot No：Q5HZJ0

Immunogen：The antiserum was produced against synthesized peptide derived from human RNase III Drosha. AA range:774-823

Specificity：RNase III Drosha Polyclonal Antibody detects endogenous levels of RNase III Drosha protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：DROSHA;RN3;RNASE3L;RNASEN;Ribonuclease 3;Protein Drosha;Ribonuclease III;RNase III;p241

Observed Band(KD)：160kD

Background：drosha ribonuclease III(DROSHA) Homo sapiens This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016],

Function：catalytic activity:Endonucleolytic cleavage to 5'-phosphomonoester.,cofactor:Magnesium or manganese.,function:Executes the initial step of microRNA (miRNA) processing in the nucleus, that is cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA.,online information:The dark side of RNA -Issue 87 of October 2007,similarity:Contains 1 DRBM (double-stranded RNA-binding) domain.,similarity:Contains 2 RNase III domains.,subcellular location:A fraction is translocated to the nucleolus during the S phase of the cell cycle.,subunit:Interacts with Sp1.,tissue specificity:Ubiquitous.,

Subcellular Location：Nucleus . Nucleus, nucleolus . A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. .

Expression：Ubiquitous.