Target：Bub3

Fields：Cell cycle;Human T-cell leukemia virus 1 infection

Gene Name：BUB3

Protein Name：Mitotic checkpoint protein BUB3

Human Gene Id：9184

Human Swiss Prot No：O43684

Mouse Gene Id：12237

Mouse Swiss Prot No：Q9WVA3

Immunogen：The antiserum was produced against synthesized peptide derived from human BUB3. AA range:279-328

Specificity：Bub3 Polyclonal Antibody detects endogenous levels of Bub3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：BUB3;Mitotic checkpoint protein BUB3

Observed Band(KD)：40kD

Background： This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

Function：function:Required for kinetochore localization of BUB1.,similarity:Belongs to the WD repeat BUB3 family.,similarity:Contains 5 WD repeats.,subunit:Interacts with BUB1 and BUBR1.,

Subcellular Location：Nucleus. Chromosome, centromere, kinetochore . Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. .

Expression： Brain,Epithelium,Placenta,Spleen,Testis,