Target：MCF2L

Gene Name：MCF2L KIAA0362

Protein Name：Guanine nucleotide exchange factor DBS (DBL's big sister) (MCF2-transforming sequence-like protein)

Human Gene Id：23263

Human Swiss Prot No：O15068

Mouse Swiss Prot No：Q64096

Rat Swiss Prot No：Q63406

Immunogen：Synthesized peptide derived from part region of human protein

Specificity：MCF2L Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：125kD

Background： This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Function：domain:The CRAL-TRIO domain is involved in interaction with inositol phospholipids.,domain:The DH domain is involved in interaction with CCPG1.,function:Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.,similarity:Belongs to the MCF2 family.,similarity:Contains 1 CRAL-TRIO domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 1 spectrin repeat.,subunit:Interacts with CCPG1, which results in specific inhibiti

Subcellular Location：[Isoform 5]: Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .; [Isoform 3]: Cytoplasm . Endomembrane system . Interaction with membranes enriched in phosphoinositides is mediated by the CRAL-TRIO domain. .; Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .

Expression： Amygdala,Brain,Muscle,PCR rescued clones,