Target：Med8

Gene Name：MED8

Protein Name：Mediator of RNA polymerase II transcription subunit 8

Human Gene Id：112950

Human Swiss Prot No：Q96G25

Mouse Gene Id：80509

Mouse Swiss Prot No：Q9D7W5

Immunogen：Synthesized peptide derived from Med8 . at AA range: 70-150

Specificity：Med8 Polyclonal Antibody detects endogenous levels of Med8 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MED8;Mediator of RNA polymerase II transcription subunit 8;Activator-recruited cofactor 32 kDa component;ARC32;Mediator complex subunit 8

Observed Band(KD)：29kD

Background： This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013],

Function：domain:The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].,function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the Mediator

Subcellular Location：Nucleus .

Expression： Cervix carcinoma,Melanoma,Testis,