Target：MCM7

Fields：DNA replication;Cell cycle

Gene Name：MCM7

Protein Name：DNA replication licensing factor MCM7

Human Gene Id：4176

Human Swiss Prot No：P33993

Mouse Gene Id：17220

Mouse Swiss Prot No：Q61881

Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human MCM7. AA range:491-540

Specificity：MCM7 Polyclonal Antibody detects endogenous levels of MCM7 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MCM7;CDC47;MCM2;DNA replication licensing factor MCM7;CDC47 homolog;P1.1-MCM3

Observed Band(KD)：81kD

Background： The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008],

Function：function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation. Required for S-phase checkpoint activation upon UV-induced damage.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,subunit:Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN.,

Subcellular Location：Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .

Expression： Brain,Cervix,Chronic myeloid leukemia cell,Lung,