Target：BIG2

Fields：Endocytosis

Gene Name：ARFGEF2

Protein Name：Brefeldin A-inhibited guanine nucleotide-exchange protein 2

Human Gene Id：10564

Human Swiss Prot No：Q9Y6D5

Mouse Gene Id：99371

Mouse Swiss Prot No：A2A5R2

Rat Gene Id：296380

Rat Swiss Prot No：Q7TSU1

Immunogen：The antiserum was produced against synthesized peptide derived from human ARFGEF2. AA range:1491-1540

Specificity：BIG2 Polyclonal Antibody detects endogenous levels of BIG2 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ARFGEF2;ARFGEP2;BIG2;Brefeldin A-inhibited guanine nucleotide-exchange protein 2;Brefeldin A-inhibited GEP 2;ADP-ribosylation factor guanine nucleotide-exchange factor 2

Observed Band(KD)：210kD

Background： ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008],

Function：disease:Defects in ARFGEF2 are the cause of autosomal recessive periventricular nodular heterotopia type 2 (PVNH2) [MIM:608097]; also called periventricular heterotopia with microcephaly autosomal recessive. PVNH2 is an autosomal recessive form characterized by microcephaly (small brain), severe developmental delay and recurrent infections. No anomalies extrinsic to the central nervous system, such as dysmorphic features or grossly abnormal endocrine or other conditions, are associated with PVNH2.,enzyme regulation:Inhibited by brefeldin A.,function:Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 SEC7 domain.,tissue specificity:Expressed in placenta, lung, heart, brain, kidney and pancreas.,

Subcellular Location：Cytoplasm. Membrane. Golgi apparatus. Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network . Endosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, dendrite . Cytoplasmic vesicle . Cell junction, synapse . Cytoplasm, cytoskeleton . Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.

Expression：Expressed in placenta, lung, heart, brain, kidney and pancreas.