Target：PNPase

Fields：RNA degradation

Gene Name：PNPT1

Protein Name：Polyribonucleotide nucleotidyltransferase 1 mitochondrial

Human Gene Id：87178

Human Swiss Prot No：Q8TCS8

Mouse Gene Id：71701

Mouse Swiss Prot No：Q8K1R3

Immunogen：The antiserum was produced against synthesized peptide derived from human PNPT1. AA range:570-619

Specificity：PNPase Polyclonal Antibody detects endogenous levels of PNPase protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PNPT1;PNPASE;Polyribonucleotide nucleotidyltransferase 1; mitochondrial;3'-5' RNA exonuclease OLD35;PNPase old-35;Polynucleotide phosphorylase 1;PNPase 1;Polynucleotide phosphorylase-like protein

Observed Band(KD)：85kD

Background： The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012],

Function：catalytic activity:RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.,function:Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction.,induction:Up-regulated in cells upon senescence and terminal differentiation. Up-regulated after treatment with interferon beta (IFN-beta).,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the polyribonucleotide nucleotidyltransferase family.,similarity:Contains 1 KH domain.,similarity:Contains 1 S1 motif domain.,subunit:Homotrimer (Potential). Interacts with TCL1A; the interaction has no effect on PNPT1 exonuclease activity.,

Subcellular Location：Cytoplasm . Mitochondrion matrix . Mitochondrion intermembrane space ; Peripheral membrane protein .

Expression： Cervix,Epithelium,Melanoma,Skin,Teratocarcinoma,Urinary bla