Target：ATG4A

Fields：Autophagy - other;Autophagy - animal

Gene Name：ATG4A

Protein Name：Cysteine protease ATG4A

Human Gene Id：115201

Human Swiss Prot No：Q8WYN0

Mouse Gene Id：666468

Mouse Swiss Prot No：Q8C9S8

Immunogen：The antiserum was produced against synthesized peptide derived from human ATG4A. AA range:81-130

Specificity：Atg4A Polyclonal Antibody detects endogenous levels of Atg4A protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ATG4A;APG4A;AUTL2;Cysteine protease ATG4A;AUT-like 2 cysteine endopeptidase;Autophagin-2;Autophagy-related cysteine endopeptidase 2;Autophagy-related protein 4 homolog A;hAPG4A

Molecular Weight(Da)：45kD

Background： Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Mar 2016],

Function：enzyme regulation:Inhibited by N-ethylmaleimide.,function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.,

Subcellular Location：Cytoplasm .

Expression： Epithelium,Kidney,Ovary,Prostate,Testis,