Target：Enolase

Fields：Glycolysis / Gluconeogenesis;Metabolic pathways;Carbon metabolism;Biosynthesis of amino acids;RNA degradation;HIF-1 signaling pathway

Gene Name：ENO2

Protein Name：Gamma-enolase

Human Gene Id：2026

Human Swiss Prot No：P09104

Mouse Gene Id：13807

Mouse Swiss Prot No：P17183

Rat Gene Id：1.00912e+008

Rat Swiss Prot No：P07323

Immunogen：The antiserum was produced against synthesized peptide derived from human NSE. AA range:371-420

Specificity：Enolase Polyclonal Antibody detects endogenous levels of Enolase protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ENO2;Gamma-enolase;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE

Observed Band(KD)：47kD

Background：enolase 2(ENO2) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008],

Function：catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.,cofactor:Magnesium. Required for catalysis and for stabilizing the dimer.,developmental stage:During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.,function:Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.,induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jacob disease.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.,similarity:Belongs to the enolase family.,subcellular location:Can translocate to the plasma membrane

Subcellular Location：Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. .

Expression：The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.