Target：Endophilin I
Fields：Endocytosis
Gene Name：SH3GL2
Protein Name：Endophilin-A1
Human Gene Id：6456
Human Swiss Prot No：Q99962
Mouse Gene Id：20404
Mouse Swiss Prot No：Q62420
Rat Gene Id：116743
Rat Swiss Prot No：O35179
Immunogen：Synthesized peptide derived from Endophilin I . at AA range: 30-110
Specificity：Endophilin I Polyclonal Antibody detects endogenous levels of Endophilin I protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：SH3GL2;CNSA2;SH3D2A;Endophilin-A1;EEN-B1;Endophilin-1;SH3 domain protein 2A;SH3 domain-containing GRB2-like protein 2
Observed Band(KD)：39kD
Background：domain:An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.,function:Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.,miscellaneous:HeLa cells expressing the N-BAR domain of SH3GL2 show tubulation of the plasma membrane. The N-BAR domain binds liposomes and induces formation of tubules from liposomes. The N-terminal amphipathic helix is required for liposome binding. The second amphipathic helix enhances liposome tubulation.,similarity:Belongs to the endophilin family.,similarity:Contains 1 BAR domain.,similarity:Contains 1 SH3 domain.,subcellular location:Concentrated in presynaptic nerve terminals in neurons.,subunit:Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with SYNJ1 and DNM1. Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation. Interacts with PDCD6IP.,tissue specificity:Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.,
Function：domain:An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.,function:Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.,miscellaneous:HeLa cells expressing the N-BAR domain of SH3GL2 show tubulation of the plasma membrane. The N-BAR domain binds liposomes and induces formation of tubules from liposomes. The N-terminal amphipathic helix is required for liposome binding. The second amphipathic helix enhances liposome tubulation.,similarity:Belongs to the endophilin family.,similarity:Contains 1 BAR domain.,similarity:Contains 1 SH3 domain.,subcel
Subcellular Location：Cytoplasm . Membrane ; Peripheral membrane protein . Early endosome . Cell junction, synapse, presynapse .
Expression：Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
