Target：Eme1

Fields：Homologous recombination;Fanconi anemia pathway

Gene Name：EME1

Protein Name：Crossover junction endonuclease EME1

Human Gene Id：146956

Human Swiss Prot No：Q96AY2

Mouse Swiss Prot No：Q8BJW7

Immunogen：Synthesized peptide derived from Eme1 . at AA range: 250-330

Specificity：Eme1 Polyclonal Antibody detects endogenous levels of Eme1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EME1;MMS4;Crossover junction endonuclease EME1;MMS4 homolog;hMMS4

Observed Band(KD)：65kD

Background： This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009],

Function：cofactor:Magnesium.,function:Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.,similarity:Belongs to the EME1/MMS4 family.,subcellular location:Recruited to regions of DNA damage in S-phase cells.,subunit:May self-associate. Interacts with MUS81. Interacts with ERCC4 and FANCM.,

Subcellular Location：Nucleus, nucleolus . Recruited to regions of DNA damage in S-phase cells.

Expression： Epithelium,Placenta,