Target：EKLF/CKLF/UKLF

Gene Name：KLF1/KLF5/KLF7

Protein Name：Krueppel-like factor 1/Krueppel-like factor 5/Krueppel-like factor 7

Human Gene Id：10661/688/8609

Human Swiss Prot No：Q13351/Q13887/O75840

Mouse Gene Id：12224/93691

Immunogen：The antiserum was produced against synthesized peptide derived from human KLF. AA range:291-340

Specificity：EKLF/CKLF/UKLF Polyclonal Antibody detects endogenous levels of EKLF/CKLF/UKLF protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：KLF1;EKLF;Krueppel-like factor 1;Erythroid krueppel-like transcription factor;EKLF;KLF5;BTEB2;CKLF;IKLF;Krueppel-like factor 5;Basic transcription element-binding protein 2;BTE-binding protein 2;Colon krueppel-like factor;GC-bo

Observed Band(KD)：38kD(EKLF) 20kD(CKLF/UKLF)

Background： This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],

Function：function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,simila

Subcellular Location：Nucleus . Colocalizes with SUMO1 in nuclear speckles. .

Expression：Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.