Target：EIF3D

Gene Name：EIF3D

Protein Name：Eukaryotic translation initiation factor 3 subunit D

Human Gene Id：8664

Human Swiss Prot No：O15371

Mouse Gene Id：55944

Mouse Swiss Prot No：O70194

Immunogen：The antiserum was produced against synthesized peptide derived from human EIF3D. AA range:101-150

Specificity：eIF3ζ Polyclonal Antibody detects endogenous levels of eIF3ζ protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EIF3D;EIF3S7;Eukaryotic translation initiation factor 3 subunit D;eIF3d;Eukaryotic translation initiation factor 3 subunit 7;eIF-3-zeta;eIF3 p66

Observed Band(KD)：64kD

Background： Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008],

Function：function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF-3 subunit D family.,subunit:Component of the eukaryotic translation initiation

Subcellular Location：Cytoplasm .

Expression： Brain,Liver,Lung,Muscle,Platelet,Skin,Uterus,