Target：eIF3α

Gene Name：EIF3J

Protein Name：Eukaryotic translation initiation factor 3 subunit J

Human Gene Id：8669

Human Swiss Prot No：O75822

Rat Gene Id：691947

Rat Swiss Prot No：A0JPM9

Immunogen：Synthesized peptide derived from eIF3α . at AA range: 40-120

Specificity：eIF3α Polyclonal Antibody detects endogenous levels of eIF3α protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EIF3J;EIF3S1;Eukaryotic translation initiation factor 3 subunit J;eIF3j;Eukaryotic translation initiation factor 3 subunit 1;eIF-3-alpha;eIF3 p35

Observed Band(KD)：30kD

Background： This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013],

Function：function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,P

Subcellular Location：Cytoplasm .

Expression： Epithelium,Fetal liver,Lung carcinoma,Ovarian carci