Target：DR4

Fields：Cytokine-cytokine receptor interaction;Viral protein interaction with cytokine and cytokine receptor;p53 signaling pathway;Apoptosis;Necroptosis;Natural killer cell mediated cytotoxicity;Pathogenic Escherichia coli infection;Salmonella infection;Influenza A;Lipid and atherosclerosis

Gene Name：TNFRSF10A

Protein Name：Tumor necrosis factor receptor superfamily member 10A

Human Gene Id：8797

Human Swiss Prot No：O00220

Immunogen：The antiserum was produced against synthesized peptide derived from human TNFRSF10A. AA range:401-450

Specificity：DR4 Polyclonal Antibody detects endogenous levels of DR4 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TNFRSF10A;APO2;DR4;TRAILR1;Tumor necrosis factor receptor superfamily member 10A;Death receptor 4;TNF-related apoptosis-inducing ligand receptor 1;TRAIL receptor 1;TRAIL-R1;CD antigen CD261

Observed Band(KD)：50kD

Background： The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008],

Function：function:Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.,similarity:Contains 1 death domain.,similarity:Contains 3 TNFR-Cys repeats.,subunit:Can interact with TRADD and RIP. Interacts with ARAP1.,tissue specificity:Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.,

Subcellular Location：Cell membrane ; Single-pass type I membrane protein . Membrane raft . Cytoplasm, cytosol . Palmitoylation is required for association with membranes. .

Expression：Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells.