Target：GCSc-γ

Fields：Cysteine and methionine metabolism;Glutathione metabolism;Metabolic pathways;Biosynthesis of cofactors;Ferroptosis

Gene Name：GCLC

Protein Name：Glutamate--cysteine ligase catalytic subunit

Human Gene Id：2729

Human Swiss Prot No：P48506

Mouse Gene Id：14629

Mouse Swiss Prot No：P97494

Rat Gene Id：25283

Rat Swiss Prot No：P19468

Immunogen：The antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315

Specificity：GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：GCLC;GLCL;GLCLC;Glutamate--cysteine ligase catalytic subunit;GCS heavy chain;Gamma-ECS;Gamma-glutamylcysteine synthetase

Observed Band(KD)：73kD

Background： Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],

Function：catalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,

Subcellular Location：cytosol,integral component of membrane,glutamate-cysteine ligase complex,

Expression： Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus,