Target：APHC

Fields：Sphingolipid metabolism;Metabolic pathways

Gene Name：ACER3

Protein Name：Alkaline ceramidase 3

Human Gene Id：55331

Human Swiss Prot No：Q9NUN7

Mouse Gene Id：66190

Mouse Swiss Prot No：Q9D099

Immunogen：The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220

Specificity：APHC Polyclonal Antibody detects endogenous levels of APHC protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ACER3;APHC;PHCA;Alkaline ceramidase 3;AlkCDase 3;Alkaline CDase 3;Alkaline dihydroceramidase SB89;Alkaline phytoceramidase;aPHC

Observed Band(KD)：35kD

Background：enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta.,

Function：enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta.,

Subcellular Location：Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein .

Expression：Ubiquitously expressed. Highly expressed in placenta (PubMed:11356846). Expressed in erythrocytes (PubMed:20207939).