Target：DNA Ligase I
Fields：DNA replication;Base excision repair;Nucleotide excision repair;Mismatch repair
Gene Name：LIG1
Protein Name：DNA ligase 1
Human Gene Id：3978
Human Swiss Prot No：P18858
Mouse Swiss Prot No：P37913
Immunogen：The antiserum was produced against synthesized peptide derived from human DNL1. AA range:111-160
Specificity：DNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：LIG1;DNA ligase 1;DNA ligase I;Polydeoxyribonucleotide synthase [ATP] 1
Observed Band(KD)：133kD
Background： This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
Function：catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG1 seem to cause immunodeficiencies and cellular hypersensitivity to DNA-damaging agents.,function:DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,online information:DNA ligase entry,online information:LIG1 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family.,
Subcellular Location：Nucleus.
Expression： Brain,Epithelium,Eye,PCR rescued clones,T lymphoblast,
