Target：DGK-ε
Fields：Glycerolipid metabolism;Glycerophospholipid metabolism;Metabolic pathways;Phosphatidylinositol signaling system;Phospholipase D signaling pathway;Choline metabolism in cancer
Gene Name：DGKE
Protein Name：Diacylglycerol kinase epsilon
Human Gene Id：8526
Human Swiss Prot No：P52429
Mouse Gene Id：56077
Mouse Swiss Prot No：Q9R1C6
Immunogen：The antiserum was produced against synthesized peptide derived from human DGKE. AA range:161-210
Specificity：DGK-ε Polyclonal Antibody detects endogenous levels of DGK-ε protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：DGKE;DAGK5;Diacylglycerol kinase epsilon;DAG kinase epsilon;Diglyceride kinase epsilon;DGK-epsilon
Observed Band(KD)：60kD
Background： Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,function:Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Expressed predominantly in testis.,
Subcellular Location：Membrane ; Single-pass membrane protein . Cytoplasm .
Expression：Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
