Target：DDX58

Fields：NF-kappa B signaling pathway;RIG-I-like receptor signaling pathway;Cytosolic DNA-sensing pathway;Hepatitis C;Hepatitis B;Measles;Influenza A;Herpes simplex virus 1 infection;Epstein-Barr virus infection;Coronavirus disease - COVID-19

Gene Name：DDX58

Protein Name：Probable ATP-dependent RNA helicase DDX58 (EC 3.6.4.13) (DEAD box protein 58) (RIG-I-like receptor 1) (RLR-1) (Retinoic acid-inducible gene 1 protein) (RIG-1) (Retinoic acid-inducible gene I protein)

Human Gene Id：23586

Human Swiss Prot No：O95786

Mouse Swiss Prot No：Q6Q899

Immunogen：Synthesized peptide derived from part region of human protein

Specificity：DDX58 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：101kD

Background： DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008],

Function：domain:The 2 CARD domains are responsible for interaction with and signaling through MAVS.,domain:The helicase domain is responsible for dsRNA recognition.,domain:The repressor domain controls homomultimerization and interaction with MAVS.,function:Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.,induction:By bacterial lipopolysaccharide (LPS) in endothelial cells. By IFN-alpha, -beta and -gamma.,PTM:Isgylated. Conjugated to ubiquitin-like protein ISG15

Subcellular Location：Cytoplasm. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell junction, tight junction. Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Associated with the actin cytoskeleton at membrane ruffles.

Expression：Present in vascular smooth cells (at protein level).