Target：DDX3Y

Gene Name：DDX3Y

Protein Name：ATP-dependent RNA helicase DDX3Y

Human Gene Id：8653

Human Swiss Prot No：O15523

Mouse Gene Id：26900

Mouse Swiss Prot No：Q62095

Immunogen：The antiserum was produced against synthesized peptide derived from human DDX3Y. AA range:41-90

Specificity：DDX3Y Polyclonal Antibody detects endogenous levels of DDX3Y protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：DDX3Y;DBY;ATP-dependent RNA helicase DDX3Y;DEAD box protein 3; Y-chromosomal

Molecular Weight(Da)：73kD

Background： The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014],

Function：disease:DDX3Y is deleted in severe non-obstructive hypospermatogenesis [MIM:400042].,function:Probable ATP-dependent RNA helicase. May play a role in spermatogenesis.,similarity:Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,subcellular location:Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.,subunit:May interact with TDRD3.,tissue specificity:Testis-specific. Expressed predominantly in spermatogonia.,

Subcellular Location：Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.

Expression：Widely expressed at the mRNA level, with highest levels in testis (PubMed:9381176). Testis-specific (at protein level). Expressed predominantly in spermatogonia, but occasionally detected in some pre-leptotene/leptotene spermatocytes (PubMed:15294876).