Target：GCK/GLK

Fields：MAPK signaling pathway

Gene Name：MAP4K2/MAP4K3

Protein Name：GCK/GLK

Human Gene Id：5871/8491

Human Swiss Prot No：Q12851/Q8IVH8

Immunogen：Synthesized peptide derived from human GCK/GLK. at AA range: 221-270

Specificity：GCK/GLK Polyclonal Antibody detects endogenous levels of GCK/GLK

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000, ELISA 1:10000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：Mitogen-activated protein kinase kinase kinase kinase 2 (EC 2.7.11.1) (B lymphocyte serine/threonine-protein kinase) (Germinal center kinase) (GC kinase) (MAPK/ERK kinase kinase kinase 2) (MEK kinase kinase 2) (MEKKK 2) (Rab8-interacting protein) Mitogen-activated protein kinase kinase kinase kinase 3 (EC 2.7.11.1) (Germinal center kinase-related protein kinase) (GLK) (MAPK/ERK kinase kinase kinase 3) (MEK kinase kinase 3) (MEKKK 3)

Observed Band(KD)：100kD

Background：mitogen-activated protein kinase kinase kinase kinase 2(MAP4K2) Homo sapiens The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.,sequence caution:Contaminating sequence. Sequence of unknown origin in the N-terminal part.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with TRAF2, MAP3K1 and RAB8A.,tissue specificity:Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.,

Subcellular Location：Cytoplasm . Basolateral cell membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein .

Expression：Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.