Target：CYP39A1

Fields：Primary bile acid biosynthesis

Gene Name：CYP39A1

Protein Name：24-hydroxycholesterol 7-alpha-hydroxylase

Human Gene Id：51302

Human Swiss Prot No：Q9NYL5

Mouse Gene Id：56050

Mouse Swiss Prot No：Q9JKJ9

Immunogen：The antiserum was produced against synthesized peptide derived from human Cytochrome P450 39A1. AA range:361-410

Specificity：CYP39A1 Polyclonal Antibody detects endogenous levels of CYP39A1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CYP39A1;24-hydroxycholesterol 7-alpha-hydroxylase;Cytochrome P450 39A1;hCYP39A1;Oxysterol 7-alpha-hydroxylase

Observed Band(KD)：54kD

Background：cytochrome P450 family 39 subfamily A member 1(CYP39A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Function：catalytic activity:(24R)-cholest-5-ene-3-beta,24-diol + NADPH + O(2) = (24R)-cholest-5-ene-3-beta,7-alpha,24-triol + NADP(+) + H(2)O.,cofactor:Heme group.,function:Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver specific.,

Subcellular Location：Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane ; Multi-pass membrane protein .

Expression：Liver specific.