Target：Cyclin A

Fields：Cell cycle;AMPK signaling pathway;Cellular senescence;Progesterone-mediated oocyte maturation;Hepatitis B;Human papillomavirus infection;Human T-cell leukemia virus 1 infection;Epstein-Barr virus infection;Pathways in cancer;Transcriptional misregulation in cancer;Viral carcinogenesis;Acute myeloid leukemia

Gene Name：CCNA1/CCNA2

Protein Name：Cyclin-A1/2

Human Gene Id：8900/890

Human Swiss Prot No：P78396/P20248

Mouse Gene Id：12427/12428

Rat Gene Id：295052

Rat Swiss Prot No：Q6AY13

Immunogen：The antiserum was produced against synthesized peptide derived from human Cyclin A. AA range:221-270

Specificity：Cyclin A Polyclonal Antibody detects endogenous levels of Cyclin A protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CCNA1;Cyclin-A1;CCNA2;CCN1;CCNA;Cyclin-A2;Cyclin-A

Observed Band(KD)：53kD

Background： The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multi

Function：developmental stage:Expression increases in early G1 phase and reaches highest levels during the S and G2/M phases.,function:May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Does not bind CDK4 and CDK5 (in vitro). The cyclin A1-CDK2 complex interacts with transcription factor E2F-1 and RB proteins. Found in a complex with CDK2, CABLES1 and CCNE1 (By similarity). Interacts with INCA1 and KLHDC9.,tissue specificit

Subcellular Location：Nucleus .

Expression：Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.