Target：CRSP130

Gene Name：MED23

Protein Name：Mediator of RNA polymerase II transcription subunit 23

Human Gene Id：9439

Human Swiss Prot No：Q9ULK4

Mouse Gene Id：70208

Mouse Swiss Prot No：Q80YQ2

Rat Swiss Prot No：Q5EB59

Immunogen：The antiserum was produced against synthesized peptide derived from human MED23. AA range:1-50

Specificity：CRSP130 Polyclonal Antibody detects endogenous levels of CRSP130 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MED23;ARC130;CRSP3;DRIP130;KIAA1216;SUR2;Mediator of RNA polymerase II transcription subunit 23;Activator-recruited cofactor 130 kDa component;ARC130;Cofactor required for Sp1 transcriptional activation subunit 3;CRSP complex subu

Observed Band(KD)：150kD

Background： The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012],

Function：alternative products:Experimental confirmation may be lacking for some isoforms,function:Required for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.,similarity:Belongs to the

Subcellular Location：Nucleus .

Expression： Brain,Cervix carcinoma,Placenta,Prostate,Testis,