Target：CNOT4
Fields：RNA degradation
Gene Name：CNOT4
Protein Name：CCR4-NOT transcription complex subunit 4
Human Gene Id：4850
Human Swiss Prot No：O95628
Mouse Gene Id：53621
Mouse Swiss Prot No：Q8BT14
Immunogen：The antiserum was produced against synthesized peptide derived from human CNOT4. AA range:61-110
Specificity：CNOT4 Polyclonal Antibody detects endogenous levels of CNOT4 protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：CNOT4;NOT4;CCR4-NOT transcription complex subunit 4;CCR4-associated factor 4;E3 ubiquitin-protein ligase CNOT4;Potential transcriptional repressor NOT4Hp
Observed Band(KD)：64kD
Background：CCR4-NOT transcription complex subunit 4(CNOT4) Homo sapiens The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010],
Function：function:Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.,pathway:Protein modification; protein ubiquitination.,PTM:Autoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Sequencing errors.,similarity:Contains 1 C3H1-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Binds CNOT1 via its C-terminus. Binds E2 ubiquitin ligases via its RING domain.,
Subcellular Location：Cytoplasm . Nucleus .
Expression： Mammary gland,Testis,
