Target：JNK1/2/3

Fields：Endocrine resistance;MAPK signaling pathway;ErbB signaling pathway;Ras signaling pathway;cAMP signaling pathway;FoxO signaling pathway;Sphingolipid signaling pathway;Mitophagy - animal;Autophagy - animal;Protein processing in endoplasmic reticulum;Apoptosis;Apoptosis - multiple species;Necroptosis;Wnt signaling pathway;Osteoclast differentiation;Focal adhesion;Tight junction;Toll-like receptor signaling pathway;NOD-like receptor signaling pathway;RIG-I-like receptor signaling pathway;C-type lectin receptor signaling pathway;IL-17 signaling pathway;Th1 and Th2 cell differentiation;Th17 cell differentiation;T cell receptor signaling pathway;Fc epsilon RI signaling pathway;TNF signaling pathway;Neurotrophin signaling pathway;Retrograde endocannabinoid signaling;Dopaminergic synapse;Inflammatory mediator regulation of TRP channels;Insulin signaling pathway;GnRH signaling pathway;Progesterone-mediated oocyte maturation;Pr

Gene Name：MAPK8/9/10

Protein Name：Mitogen-activated protein kinase 8/9/10

Human Gene Id：5599/5601/5602

Human Swiss Prot No：P45983/P45984/P53779

Mouse Gene Id：26419/26420

Rat Gene Id：116554/50658/25272

Rat Swiss Prot No：P49185/P49186/P49187

Immunogen：The antiserum was produced against synthesized peptide derived from human SAPK/JNK. AA range:166-215

Specificity：JNK1/2/3 Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MAPK8;JNK1;PRKM8;SAPK1;SAPK1C;Mitogen-activated protein kinase 8;MAP kinase 8;MAPK 8;JNK-46;Stress-activated protein kinase 1c;SAPK1c;Stress-activated protein kinase JNK1;c-Jun N-terminal kinase 1;MAPK9;JNK2;PRKM9;SAPK1A;Mi

Observed Band(KD)：46kD,54kD

Background： The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spl

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.,function:JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.,function:Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP

Subcellular Location：Cytoplasm . Nucleus . Cell junction, synapse . In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity). Colocalizes with POU5F1 in the nucleus. .

Expression： Brain,Epithelium,Fetal brain,Lung,Pooled,Testis,