Target：ILKAP

Gene Name：ILKAP

Protein Name：Integrin-linked kinase-associated serine/threonine phosphatase 2C

Human Gene Id：80895

Human Swiss Prot No：Q9H0C8

Mouse Gene Id：67444

Mouse Swiss Prot No：Q8R0F6

Rat Gene Id：64538

Rat Swiss Prot No：Q9Z1Z6

Immunogen：The antiserum was produced against synthesized peptide derived from human ILKAP. AA range:41-90

Specificity：ILKAP Polyclonal Antibody detects endogenous levels of ILKAP protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ILKAP;Integrin-linked kinase-associated serine/threonine phosphatase 2C;ILKAP

Observed Band(KD)：45kD

Background： The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. [provided by RefSeq, Jul 2008],

Function：catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 2 magnesium or manganese ions per subunit.,function:Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.,induction:Inhibited rather than stimulated by Magnesium.,similarity:Belongs to the PP2C family.,similarity:Contains 1 PP2C-like domain.,subunit:Interacts with ILK. Specific association with ILK is independent of the catalytic activity of either partner.,tissue specificity:Widely expressed. Highest levels expressed in striated muscle. Muc

Subcellular Location：Cytoplasm .

Expression：Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.