Target：FIR

Fields：Rap1 signaling pathway;Adherens junction

Gene Name：FARP2

Protein Name：FERM RhoGEF and pleckstrin domain-containing protein 2

Human Gene Id：9855

Human Swiss Prot No：O94887

Mouse Gene Id：227377

Mouse Swiss Prot No：Q91VS8

Immunogen：The antiserum was produced against synthesized peptide derived from human FIR. AA range:331-380

Specificity：FIR Polyclonal Antibody detects endogenous levels of FIR protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：FARP2;KIAA0793;PLEKHC3;FERM; RhoGEF and pleckstrin domain-containing protein 2;FERM domain including RhoGEF;FIR;Pleckstrin homology domain-containing family C member 3;PH domain-containing family C member 3

Observed Band(KD)：119kD

Background：function:Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 FERM domain.,similarity:Contains 2 PH domains.,subunit:Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding.,

Function：function:Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 FERM domain.,similarity:Contains 2 PH domains.,subunit:Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding.,

Subcellular Location：cytoplasm,cytosol,cytoskeleton,extrinsic component of membrane,

Expression： Brain,Testis,