Target：ERdj3

Fields：Protein processing in endoplasmic reticulum

Gene Name：DNAJB11

Protein Name：DnaJ homolog subfamily B member 11

Human Gene Id：51726

Human Swiss Prot No：Q9UBS4

Mouse Gene Id：67838

Mouse Swiss Prot No：Q99KV1

Rat Gene Id：360734

Rat Swiss Prot No：Q6TUG0

Immunogen：The antiserum was produced against synthesized peptide derived from human DNAJB11. AA range:31-80

Specificity：ERdj3 Polyclonal Antibody detects endogenous levels of ERdj3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：DNAJB11;EDJ;ERJ3;HDJ9;PSEC0121;DnaJ homolog subfamily B member 11;APOBEC1-binding protein 2;ABBP-2;DnaJ protein homolog 9;ER-associated DNAJ;ER-associated Hsp40 co-chaperone;ER-associated dnaJ protein 3;ERdj3;ERj3p;HEDJ;Human

Observed Band(KD)：40kD

Background： This gene encodes a soluble glycoprotein of the endoplasmic reticulum (ER) lumen that functions as a co-chaperone of binding immunoglobulin protein, a 70 kilodalton heat shock protein chaperone required for the proper folding and assembly of proteins in the ER. The encoded protein contains a highly conserved J domain of about 70 amino acids with a characteristic His-Pro-Asp (HPD) motif and may regulate the activity of binding immunoglobulin protein by stimulating ATPase activity. [provided by RefSeq, Mar 2014],

Function：caution:PubMed:11584023 reported a cytosolic, as well as nuclear subcellular location. This result was obtained using an N-terminally GFP-tagged construct which most probably affected signal peptide-driven targeting to the ER. As a consequence, the in vivo revelance of the observed interaction with APOBEC1, a nuclear protein, is dubious. This holds true for the interaction with PWP1.,function:Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.,induction:By ER stress-inducing agents, such as thapsigargin and tunicamycin.,PTM:Contains high-mannose Endo H-sensitive carbohydrates.,PTM:Cys-169, Cys-171, Cys-193 and Cys-196

Subcellular Location：Endoplasmic reticulum lumen . Associated with the ER membrane in a C-terminally epitope-tagged construct.

Expression：Widely expressed.