Target：NPRL2

Fields：mTOR signaling pathway

Gene Name：NPRL2

Protein Name：Nitrogen permease regulator 2-like protein

Human Gene Id：10641

Human Swiss Prot No：Q8WTW4

Mouse Gene Id：56032

Mouse Swiss Prot No：Q9WUE4

Immunogen：Synthesized peptide derived from the C-terminal region of human NPRL2.

Specificity：NPRL2 Polyclonal Antibody detects endogenous levels of NPRL2 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NPRL2;TUSC4;Nitrogen permease regulator 2-like protein;NPR2-like protein;Gene 21 protein;G21 protein;Tumor suppressor candidate 4

Observed Band(KD)：43kD

Background：function:Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs.,similarity:Belongs to the NPR2 family.,subunit:Interacts with PDPK1.,tissue specificity:Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested.,

Function：function:Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs.,similarity:Belongs to the NPR2 family.,subunit:Interacts with PDPK1.,tissue specificity:Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested.,

Subcellular Location：Lysosome membrane . Localization to lysosomes is amino acid-independent. .

Expression：Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes (PubMed:27173016, PubMed:26505888). Expressed in most lung cancer cell lines tested.