Target：NIPP1

Gene Name：PPP1R8

Protein Name：Nuclear inhibitor of protein phosphatase 1

Human Gene Id：5511

Human Swiss Prot No：Q12972

Mouse Gene Id：100336

Mouse Swiss Prot No：Q8R3G1

Immunogen：The antiserum was produced against synthesized peptide derived from human PPP1R8. AA range:196-245

Specificity：NIPP1 Polyclonal Antibody detects endogenous levels of NIPP1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PPP1R8;ARD1;NIPP1;Nuclear inhibitor of protein phosphatase 1;NIPP-1;Protein phosphatase 1 regulatory inhibitor subunit 8

Observed Band(KD)：40kD

Background： This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008],

Function：cofactor:Magnesium. Endoribonuclease function is magnesium-dependent.,domain:Has a basic N- and C-terminal and an acidic central domain.,function:Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.,function:Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.,miscellaneous:A synthetic peptide, NIPP-1(330-351), is able to inhibit PP-1. Phosphorylation of Tyr-335 reduces PP-1 inhibition, whereas phosphorylation

Subcellular Location：Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear.; [Isoform Gamma]: Cytoplasm. Found mainly in the cytoplasm.

Expression：Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.